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Fluorexon: A Strategic Imperative for Advanced Bio-Imaging and Material Science In the rapidly evolving landscapes of biotechnology, life sciences, and advanced materials, the demand for highly sensitive and reliable fluorescent indicators has never been greater. Fluorexon , also known by its CAS number 1461-15-0, stands out as a pivotal compound, offering unparalleled performance across a spectrum of critical applications. Its unique fluorescent properties make it indispensable for precise measurements, detection, and visualization in complex systems. This article delves into the technical supremacy of this compound, its manufacturing excellence, diverse applications, and the inherent advantages it offers to B2B professionals seeking to push the boundaries of scientific discovery and industrial innovation. The increasing complexity of research and development in areas such as cellular biology, environmental monitoring, and diagnostic assays necessitates fluorophores that deliver consistent, high-fidelity results. The industry trend is moving towards reagents that combine high quantum yield with excellent photostability and biocompatibility. Fluorexon is engineered to meet these rigorous demands, providing a robust solution for enhancing assay sensitivity and improving data accuracy. Its integration into various platforms represents a significant leap forward for analytical chemistry and biochemical research, demonstrating its crucial role in modern scientific methodologies. The Meticulous Manufacturing Process of Fluorexon The production of high-purity Fluorexon (CAS: 1461-15-0) is a testament to sophisticated chemical engineering and stringent quality control. Our manufacturing process begins with the careful selection of high-grade raw materials, ensuring a foundation of purity essential for the synthesis of a reliable fluorescent indicator. The primary synthesis involves a multi-step organic reaction, precisely controlled for temperature, pressure, and reactant ratios to maximize yield and minimize impurities. This critical phase is monitored continuously using advanced analytical techniques, including High-Performance Liquid Chromatography (HPLC) and Nuclear Magnetic Resonance (NMR) spectroscopy, to ensure the formation of the desired molecular structure and prevent the generation of unwanted byproducts. Following the synthesis, the crude product undergoes an extensive purification regimen. This typically involves a combination of recrystallization, solvent extraction, and advanced chromatographic separation methods to remove unreacted starting materials, intermediates, and any trace impurities. Each purification step is validated against rigorous internal specifications to ensure exceptional purity levels, often exceeding 98%. Post-purification, the Fluorexon is subjected to comprehensive quality assurance testing, adhering to international standards such as ISO 9001 and relevant chemical industry benchmarks like those set by ASTM for analytical reagents. This meticulous approach guarantees a product with consistent performance, a long shelf-life, and suitability for the most demanding applications in fields like chemical analysis, environmental science, and life sciences. The final product undergoes rigorous validation for its spectroscopic properties, including excitation and emission maxima, quantum yield, and extinction coefficient, to confirm its suitability for intended fluorescent applications. Furthermore, stability studies are conducted to predict its long-term performance under various storage conditions. This holistic manufacturing and testing protocol ensures that every batch of Fluorexon delivered maintains its high sensitivity and specificity, providing users with a dependable reagent critical for accurate and repeatable experimental outcomes across diverse industries, from academic research to industrial quality control. Technical Specifications and Performance Metrics Understanding the precise technical parameters of Fluorexon (1461-15-0) is crucial for its effective integration into research and industrial processes. The compound's molecular structure affords specific spectroscopic characteristics that are leveraged in its applications. Below is a detailed table outlining key technical specifications derived from rigorous testing and analysis, providing a clear reference for potential applications and compatibility. These parameters underscore the compound's high purity and consistent performance, essential for achieving reliable experimental results and maintaining industrial process integrity. Parameter Specification Method/Note CAS Number 1461-15-0 Chemical Abstracts Service Registry Number Molecular Formula C20H12O7 Calculated from elemental analysis Molecular Weight 364.30 g/mol Theoretical value Purity (HPLC) ≥ 98.0% High-Performance Liquid Chromatography Appearance Orange to Red Powder Visual inspection Excitation Max (λex) 490 nm (in alkaline solution) Spectrophotometric analysis Emission Max (λem) 515 nm (in alkaline solution) Spectrophotometric analysis Solubility Soluble in dilute NaOH, DMSO; slightly soluble in water Empirical observation Storage Temperature 2-8 °C, protected from light Recommended conditions for stability These specifications highlight Fluorexon 's suitability as a sensitive fluorescent probe. Its high purity ensures minimal interference from contaminants, which is paramount in sensitive biochemical assays. The defined excitation and emission profiles allow for precise instrument calibration and selective detection in multi-labeling experiments. Furthermore, its solubility characteristics dictate appropriate solvent systems for diverse application environments, making it a versatile tool for researchers and manufacturers alike. Key Application Scenarios and Technical Advantages Fluorexon 's versatility extends across a broad spectrum of critical applications, offering distinct technical advantages over conventional indicators. In the field of metal ion detection, Fluorexon is widely recognized as a highly sensitive fluorescent chelating agent for various metal ions, including calcium, magnesium, and zinc. Its ability to undergo a significant change in fluorescence intensity upon binding to these ions makes it an invaluable tool for environmental monitoring, industrial water treatment, and biological studies of cellular ion dynamics. Its superior selectivity and sensitivity reduce the need for complex sample preparation, thereby streamlining analytical workflows and enhancing efficiency. Beyond ion detection, Fluorexon (CAS: 1461-15-0) finds significant utility in enzyme assays, particularly for esterase activity, where its non-fluorescent ester form is hydrolyzed to produce the highly fluorescent free acid. This enables real-time monitoring of enzymatic reactions with high spatial and temporal resolution, crucial for drug discovery and biochemical pathway elucidation. In cell biology, it is employed for viability assays and as a pH indicator within living cells due to its pH-dependent fluorescence properties, providing critical insights into cellular physiology without causing significant cytotoxicity. The compound's intrinsic stability and excellent quantum yield contribute to its reliability, minimizing signal degradation and maximizing data integrity in long-term experiments or continuous monitoring systems. For industries such as pharmaceuticals and diagnostics, the high purity and batch-to-batch consistency of Fluorexon are paramount. Our product ensures reproducible results across multiple experiments and production batches, a critical factor for regulatory compliance and product reliability. Unlike some alternative fluorophores that may suffer from rapid photobleaching or pH sensitivity, Fluorexon demonstrates enhanced photostability and a robust response profile, leading to more reliable data and reduced experimental variability. This translates into tangible benefits, including reduced reagent consumption, minimized retesting, and accelerated research and development cycles. Why Partner for Your Fluorexon Needs? Choosing the right supplier for critical chemical reagents like Fluorexon is a strategic decision that impacts the success of your projects. Our commitment to quality is underscored by our ISO 9001 certified manufacturing facilities and adherence to strict cGMP (current Good Manufacturing Practices) principles where applicable, ensuring that every batch of Fluorexon (CAS: 1461-15-0) meets or exceeds industry expectations. We boast extensive experience in synthesizing and purifying complex organic compounds, with a dedicated team of Ph.D. chemists and quality control specialists overseeing every stage of production. This deep expertise allows us to offer not just a product, but a solution optimized for high performance and reliability. Our service model includes flexible customization options for Fluorexon , ranging from specific purity grades to tailored packaging sizes and bulk supply, to meet the unique requirements of your research or manufacturing scale. We understand that each client's needs are distinct, and our technical support team works closely with you to develop optimal solutions. For example, a recent collaboration with a major diagnostic company involved providing a customized batch of Fluorexon with enhanced aqueous solubility and a specific pH-response profile, which significantly improved the performance of their immunoassay kit, leading to a 15% increase in assay sensitivity and reducing detection limits. Our proactive client support and rapid response times ensure minimal disruption to your operations. In terms of reliability and supply chain integrity, our robust logistics network ensures prompt and secure delivery of Fluorexon globally. We offer transparent communication regarding lead times and delivery schedules, typically ranging from 7-14 business days for standard orders, with expedited options available upon request. Our quality assurance extends beyond product specifications to comprehensive documentation, including Certificates of Analysis (CoA) and Material Safety Data Sheets (MSDS), guaranteeing full traceability and regulatory compliance. With over a decade of dedicated service in the specialty chemical sector, we have built a reputation for trust and excellence, affirmed by long-standing partnerships with leading pharmaceutical companies, academic institutions, and research organizations worldwide. Frequently Asked Questions (FAQ) about Fluorexon Q1: What is the primary use of Fluorexon ? A1: Fluorexon is primarily used as a fluorescent indicator for metal ions (especially calcium, magnesium, zinc) and in enzyme assays, particularly for esterase activity, as well as a pH indicator and cell viability probe in biological systems. Q2: What is the recommended storage condition for Fluorexon ? A2: We recommend storing Fluorexon at 2-8 °C, protected from light, in a tightly sealed container111 to maintain its stability and fluorescence properties over an extended period. Q3: How does the purity of Fluorexon impact its performance? A3: High purity (≥ 98.0% by HPLC) is critical. Impurities can cause background fluorescence, reduce sensitivity, or interfere with specific binding, leading to inaccurate results. Our stringent purification processes ensure optimal performance. Q4: Can Fluorexon be customized for specific applications? A4: Yes, we offer customized synthesis and packaging options for Fluorexon (CAS: 1461-15-0), including different purity grades, specific formulations, or bulk quantities to meet unique research or manufacturing requirements. Please contact our technical sales team for a consultation. Conclusion: Pioneering Innovation with Fluorexon The strategic adoption of high-quality Fluorexon (CAS: 1461-15-0) is essential for any entity operating at the forefront of chemical analysis, biological imaging, or industrial diagnostics. Its multifaceted utility, combined with our rigorous manufacturing standards and commitment to customer support, establishes it as the premier choice for achieving precise, reliable, and reproducible results. As industries continue to demand more sophisticated and sensitive analytical tools, Fluorexon stands ready to empower researchers and developers to overcome current challenges and unlock new possibilities. Partner with us to leverage the full potential of this exceptional fluorescent probe in your next groundbreaking project. References Analytical Chemistry Journal Journal of Biological Chemistry Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy Applied Materials Today Biochemical and Biophysical Research Communications